A mouse model of fenugreek allergy
نویسندگان
چکیده
Methods Female C3H/HeJ mice were gavaged at days 0, 1, 2, 7, and 21 with fenugreek in doses ranging from 0.1 mg to 10 mg. Cholera toxin (CT) was used as an adjuvant (10 μg/mouse). The mice were euthanized on day 28, and sera were analysed for fenugreek-specific IgE using the heterologous passive cutaneous anaphylaxis assay. Based on this dose-response experiment, twenty four mice were gavaged with the optimal dose of fenugreek (4.2 mg) and CT at days 0, 1, 2, 7, 21 and 28. Eight mice were gavaged with CT only and 8 mice were not treated. At day 35 all mice received a large dose of fenugreek (25.0 mg) and clinical reactions were observed.
منابع مشابه
بررسی تأثیر دانهی شنبلیله و بذر کتان برگُرگرفتگی حاصل از یائسگی
Abstract Background : Hot flush is the most common problem in menopausal women. One of the alternative treatments is phytostrogens. So this study aimed to examine the effects of fenugreek and flaxseeds on frequency and severity of hot flush. Materials & Methods: In this randomized controlled clinical trials the sample population was 60 women with hot flush who referred to urbon heath cent...
متن کاملP83: Central Neuropathic Pain Development in Experimental Autoimmune Encephalomyelitis C57BL/6 Mouse Model Induced by QS-21 Adjuvant
Central neuropathic pain (CNP) is considered as a complicated sensory disturbance which many multiple sclerosis (MS) patients suffer from. Although monophasic experimental autoimmune encephalomyelitis (EAE) mouse model is a gold standard model in preclinical research of MS, severe movement deficit could confound pain behaviors evaluation over the disease course. In this study, complete Freund&r...
متن کاملA Review on the Functional Properties, Nutritional Content, Medicinal Utilization and Potential Application of Fenugreek
Fenugreek is one of the well known spices in human food. Its seeds and green leaves are used in food as well as in medicinal application which is an old practice of human history. It provides natural food fibre and other nutrients required in human body [1]. Fenugreek has strong spicy and seasoning type sweet flavor [2]. Aromatic and flavourful fenugreek is a popular spice and is widely used fo...
متن کاملEvaluation of Fenugreek (Trigonella foenum-graceum L.), Effects Seeds Extract on Insulin Resistance in Women with Polycystic Ovarian Syndrome
PCOS (Polycystic Ovarian Syndrome) is associated with insulin resistance, obesity and disorders of lipid metabolism as well as infertility. Fenugreek seeds extract is successfully used in lowering blood glucose. Metformin has also the same effect but in a different way. The aim of this study was the assessment of fenugreek effects on insulin resistance in women with PCOS.This was a prospective ...
متن کاملEvaluation of Fenugreek (Trigonella foenum-graceum L.), Effects Seeds Extract on Insulin Resistance in Women with Polycystic Ovarian Syndrome
PCOS (Polycystic Ovarian Syndrome) is associated with insulin resistance, obesity and disorders of lipid metabolism as well as infertility. Fenugreek seeds extract is successfully used in lowering blood glucose. Metformin has also the same effect but in a different way. The aim of this study was the assessment of fenugreek effects on insulin resistance in women with PCOS.This was a prospective ...
متن کامل